Simple Plex Mouse Adiponectin Cartridge
R&D Systems | Catalog # SPCKB-MP-008025
Customize My Cartridge on Bio-Techne
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse Adiponectin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 37 | 37 |
| Mean (pg/mL) | 83.0 | 4336 | 86.5 | 4358 |
| Standard Deviation | 2.80 | 187 | 9.23 | 278 |
| CV% | 3.4 | 4.3 | 10.7 | 6.4 |
Recovery for Simple Plex Mouse Adiponectin Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 109 | 107-110 |
Linearity
Samples containing and/or spiked with high concentrations of mouse Adiponectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse Adiponectin Cartridge
Mouse Adiponectin/ACRP30 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Adiponectin/Acrp30
Adiponectin (Acrp30) is secreted primarily by adipocytes and induces multiple paracrine and endocrine effects on metabolism and inflammation. Adiponectin promotes insulin sensitivity, decreased serum glucose and triglyceride levels, and increased serum glucagon levels. It promotes fatty acid uptake and oxidation, glucose uptake, and lactate production in skeletal muscle. Adiponectin promotes the development of M2 macrophages and promotes the C1qRp mediated clearance of apoptotic cell debris. Decreased plasma HMW Adiponectin levels are associated with upper body obesity, insulin resistance, reduced fatty acid oxidation, dyslipidemia, coronary artery disease, and atherogenesis. Adiponectin forms homotrimers, hexamers, and high molecular weight (HMW) complexes and signals through the receptors AdipoR1 and AdipoR2.
Alternate Names
Gene Symbol
Additional Adiponectin/Acrp30 Products
Product Documents for Simple Plex Mouse Adiponectin Cartridge
Product Specific Notices for Simple Plex Mouse Adiponectin Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Mouse Adiponectin Cartridge
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