Simple Plex Mouse CCL4/MIP-1 beta Cartridge

R&D Systems | Catalog # SPCKB-MP-003557

Simple Plex Mouse CCL4/MIP-1 beta assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.2 pg/mL

Assay Range

1.26-1920 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse CCL4/MIP-1 beta Cartridge Summary

Simple Plex assay for the detection of mouse MIP-1 beta in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse MIP-1 beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 102 99
Mean (pg/mL) 13.2 614 14.4 684
Standard Deviation 0.832 60.4 1.18 79.3
CV% 6.3 9.8 8.2 11.6

Recovery for Simple Plex Mouse CCL4/MIP-1 beta Cartridge

Recovery was not necessary as MIP-1 beta shows five points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse MIP-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Mouse CCL4/MIP-1b Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Mouse CCL4/MIP-1 beta Cartridge

Mouse CCL4/MIP-1b Simple Plex Assay Standard Curve

Mouse CCL4/MIP-1 beta Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL4/MIP-1 beta

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta

Entrez Gene IDs

6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)

Gene Symbol

CCL4

Additional CCL4/MIP-1 beta Products

Product Documents for Simple Plex Mouse CCL4/MIP-1 beta Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse CCL4/MIP-1 beta Cartridge

For research use only

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