Simple Plex Mouse GM-CSF Cartridge

Name: Simple Plex Mouse GM-CSF Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-003559
Price: 600 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-003559

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Product Documents

Simple Plex Mouse GM-CSF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.2 pg/mL

Assay Range

0.63-6040 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse GM-CSF Cartridge Summary

Simple Plex assay is for the detection of mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GM-CSF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse GM-CSF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GM-CSF assay.

Inter-Assay Precision (Precision between assays) Replicates of each GM-CSF control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse GM-CSF.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	99	98
Mean (pg/mL)	24.9	1518	35.9	1727
Standard Deviation	2.07	75.6	2.97	145
CV%	5.9	5.0	8.3	8.4

Recovery for Simple Plex Mouse GM-CSF Cartridge

Recovery for the mouse GM-CSF assays was evaluated at three different spiked concentrations of GM-CSF protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	106	101-110
Heparin Plasma (n=4)	103	93-110
Serum (n=4)	99	97-106

Linearity

Samples containing and/or spiked with high concentrations of mouse GM-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse GM-CSF Cartridge

Simple Plex Mouse GM-CSF Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse GM-CSF Assay. Assay range is 0.63-6040 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.