Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge
R&D Systems | Catalog # SPCKB-MP-010216
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 56 | 55 |
| Mean (pg/mL) | 13.3 | 628 | 11.9 | 570 |
| Standard Deviation | 0.36 | 15.6 | 0.70 | 37.7 |
| CV% | 2.7 | 2.5 | 5.9 | 6.6 |
Recovery for Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 110-112 |
Linearity
Samples containing and/or spiked with high concentrations of Flt-3 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge
Mouse/Rat Flt-3 Ligand/FLT3L Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Flt-3 Ligand/FLT3L
Flt-3 Ligand, also known as FLT3L, is a cytokine that promotes the differentiation or expansion of multiple hematopoietic cell lineages including monocytes, immature dendritic cells, and early B cell lineage cells. It synergizes with other cytokines to promote the myeloid differentiation and mobilization of hematopoietic stem cells, to induce NK cell development, and to induce terminal B cell maturation. Flt-3 Ligand/FLT3L is expressed as a dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand/FLT3L signal through the tyrosine kinase receptor Flt-3/Flk-2.
Long Name
Alternate Names
Gene Symbol
Additional Flt-3 Ligand/FLT3L Products
Product Documents for Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Mouse/Rat Flt-3 Ligand/FLT3L Cartridge
For research use only
Related Research Areas
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