Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Name: Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-008305
Price: 885 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-008305

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Product Documents

Simple Plex Mouse/Rat VCAM-1/CD106 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.59 pg/mL

Assay Range

7.97-30372 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Vascular Cell Adhesion Molecule 1 (VCAM-1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all VCAM-1/CD106 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural and recombinant mouse and rat VCAM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VCAM-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each VCAM-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for VCAM-1.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	44	43
Mean (pg/mL)	197	9548	198	10414
Standard Deviation	8.38	546	21.2	1216
CV%	4.2	5.7	10.7	11.7

Recovery for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Recovery for the mouse/rat VCAM-1 assays was evaluated at three different spiked concentrations of VCAM-1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	105	103-107

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat VCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Simple Plex Mouse/Rat VCAM-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat VCAM-1 Assay. Assay range is 7.97-30,372 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VCAM-1/CD106

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.