Sirtuin 3/SIRT3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87037

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sirtuin 3/SIRT3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037] - Staining of human skin shows strong cytoplasmic positivity in the basal layer of epidermal cells.
Sirtuin 3/SIRT3 Antibody - BSA Free Western Blot: Sirtuin 3/SIRT3 Antibody - BSA Free [NBP1-87037]

Western Blot: Sirtuin 3/SIRT3 Antibody - BSA Free [NBP1-87037]

Analysis in control (vector only transfected HEK293T lysate) and SIRT3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for Sirtuin 3/SIRT3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Entrez Gene IDs

23410 (Human)

Gene Symbol

SIRT3

UniProt

Q9NTG7 (Human)

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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