SLC28A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-30857

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MENDPSRRRESISLTPVAKGLENMGADFLESLEEGQLPRSDLSPAEIRSSWSEAAPKPFSRWRNLQPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLC28A1 Antibody - BSA Free

Western Blot: SLC28A1 Antibody [NBP2-30857]

Western Blot: SLC28A1 Antibody [NBP2-30857]

Western Blot: SLC28A1 Antibody [NBP2-30857] - Staining of human small intestine shows high expression.
Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining in human small intestine and tonsil tissues using anti-SLC28A1 antibody. Corresponding SLC28A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human kidney shows membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857]

Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human tonsil shows low expression as expected.
SLC28A1 Antibody

Immunohistochemistry: SLC28A1 Antibody [NBP2-30857] -

Immunohistochemistry: SLC28A1 Antibody [NBP2-30857] - PDAC PDX models of acquired gemcitabine resistance harbored changes in expression of some gemcitabine transporters & metabolizing enzymes. Tumor tissue harvested on days 190, 120, & 179 for PA4.gemR, PA10.gemR & PA16.gemR, respectively, was immunostained for RRM1, RRM2, hCNT1, hENT1, CDA, & dCK. Tumor tissue harvested from control mice bearing gemcitabine-naïve PA4, PA10 or PA16 tumors served as controls for IHC staining experiments. Scale bar = 10 μm. Quantitation of IHC data in A is shown as expression indices below each photomicrograph (A); protein expression in gemR models that were developed by exposure to drug in vivo, compared to their respective parent tumor models. Each protein has been reported to be associated with gemcitabine resistance in models developed in vitro. ND: no difference between gemcitabine-naïve control & gemR tumors. Values in the Table were calculated by dividing the EI of each protein determined by IHC expressed by gemR/parent (control) tumors. A change of > 1.5-fold was interpreted as a real increase or decrease (B). PDAC: pancreatic ductal adenocarcinoma; PDX: patient-derived xenograft; gemR: gemcitabine resistance; IHC: immunohistochemistry Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33073205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SLC28A1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC28A1

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine

Alternate Names

CNT 1, CNT1HCNT1, Concentrative nucleoside transporter 1, hCNT1, Na(+)/nucleoside cotransporter 1, sodium/nucleoside cotransporter 1, Sodium-coupled nucleoside transporter 1, solute carrier family 28 (sodium-coupled nucleoside transporter), member 1, Solute carrier family 28 member 1

Gene Symbol

SLC28A1

UniProt

Additional SLC28A1 Products

Product Documents for SLC28A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLC28A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLC28A1 Antibody - BSA Free

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Protocols

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