SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43803

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3123

Format

Azide and BSA Free
View all SLC6A3/DAT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q01959, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of human caudate nucleus shows strong positivity in dopaminergic fibers.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of human cerebral cortex shows weak positivity in a subset of neuronal processes.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of human liver shows no positivity in hepatocytes as expected.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of rat caudate putamen shows moderate positivity in dopaminergic fibers.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of mouse caudate putamen shows moderate positivity in dopaminergic fibers.
SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody [NBP3-43803]

Staining of human substantia nigra shows strong cytoplasmic positivity in dopaminergic neurons.

Applications for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC6A3/DAT1

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Long Name

Dopamine Transporter

Alternate Names

DAT1, SLC6A3, VNTR

Gene Symbol

SLC6A3

Additional SLC6A3/DAT1 Products

Product Documents for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SLC6A3/DAT1 Antibody (CL3123) - Azide and BSA Free

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  • Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?

    A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

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