Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 710305
View all Slit2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
Gln26-Gln900
Accession # Q9R1B9

Specificity

Stains human and mouse Slit2 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Slit2 Antibody (710305) [DyLight 755]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Slit2

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Gene Symbol

SLIT2

Additional Slit2 Products

Product Documents for Slit2 Antibody (710305) [DyLight 755]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Slit2 Antibody (710305) [DyLight 755]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Slit2 Antibody (710305) [DyLight 755]

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    • A: The literature states that Slit2 undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. The immunogen sequence used for the antibody is confined to the N-term fragment sequence so we would expect this antibody to bind only the N-term fragment after cleavage. 
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