Human/Mouse Slit2 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Stains human and mouse Slit2 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Recombinant Monoclonal Rat IgG2B Clone # 710305
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
    Gln26-Gln900
    Accession # Q9R1B9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with Human Slit2 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with human Slit2 was stained with Rat Anti-Mouse Slit2 Monoclonal Antibody (Catalog # MAB5444, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with Mouse Slit2 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse Slit2 was stained with Rat Anti-Mouse Slit2 Monoclonal Antibody (Catalog # MAB5444, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Slit2
Slit2 is a 180-210 kDa secreted member of the SLIT family of proteins. It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulose lutein cells, and likely keratinocytes. Slit2 binds to multiple receptors, including ROBO-1 thru -4, Laminin-1, Dan, Gremlin and Netrin-1. Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion. Mature mouse Slit2 is 1496 amino acids (aa) in length (aa 26-1521). It contains multiple intermingled domains, including nine EGF-like domains, 20 Leu-rich repeats (LRRs), one laminin G-like and CTCK (C-terminal Cys knot-like) domain, and eight total C- plus N-terminal LRRs. There are two potential isoform splice variants. One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021. Slit2 apparently undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. This processing does not inactivate Slit2. Rather, it creates molecules with distinct activities. For example, the N-terminal fragment will bind ROBO1 and repel motor axon migration, while the C-terminal fragment won't bind ROBO1, but will bind glypican-1 and promote motor axon migration. Over aa 26-900, mouse Slit2 shares 99% and 97% aa sequence identity with rat and human Slit2, respectively.
  • Long Name:
    SLIT Homolog 2
  • Entrez Gene IDs:
    9353 (Human); 20563 (Mouse); 360272 (Rat)
  • Alternate Names:
    FLJ14420; SLIL3; slit (Drosophila) homolog 2; slit homolog 2 (Drosophila); slit homolog 2 protein; Slit2; Slit-2SLIL3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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