Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 710305
View all Slit2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
Gln26-Gln900
Accession # Q9R1B9

Specificity

Stains human and mouse Slit2 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse Slit2 Antibody

Detection of Slit2 antibody in CHO Chinese Hamster Cell Line Transfected with Human Slit2 antibody by Flow Cytometry.

Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with Human Slit2 by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with human Slit2 was stained with Rat Anti-Mouse Slit2 Monoclonal Antibody (Catalog # MAB5444, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Detection of Slit2 antibody in CHO Chinese Hamster Cell Line Transfected with Mouse Slit2 antibody by Flow Cytometry.

Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with Mouse Slit2 by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with mouse Slit2 was stained with Rat Anti-Mouse Slit2 Monoclonal Antibody (Catalog # MAB5444, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Human/Mouse Slit2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with human or mouse Slit2

Reviewed Applications

Read 1 review rated 5 using MAB5444 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Slit2

Slit2 is a 180-210 kDa secreted member of the SLIT family of proteins. It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulose lutein cells, and likely keratinocytes. Slit2 binds to multiple receptors, including ROBO-1 thru -4, Laminin-1, Dan, Gremlin and Netrin-1. Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion. Mature mouse Slit2 is 1496 amino acids (aa) in length (aa 26-1521). It contains multiple intermingled domains, including nine EGF-like domains, 20 Leu-rich repeats (LRRs), one laminin G-like and CTCK (C-terminal Cys knot-like) domain, and eight total C- plus N-terminal LRRs. There are two potential isoform splice variants. One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021. Slit2 apparently undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. This processing does not inactivate Slit2. Rather, it creates molecules with distinct activities. For example, the N-terminal fragment will bind ROBO1 and repel motor axon migration, while the C-terminal fragment won't bind ROBO1, but will bind glypican-1 and promote motor axon migration. Over aa 26-900, mouse Slit2 shares 99% and 97% aa sequence identity with rat and human Slit2, respectively.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

Q9R1B9 (Mouse)

Additional Slit2 Products

Product Documents for Human/Mouse Slit2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Slit2 Antibody

For research use only

Customer Reviews for Human/Mouse Slit2 Antibody (1)

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  • Human/Mouse Slit2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA format in combination with pAb (AF5444)) and protein (5444-SL). This combination works well for detecting SLIT2 in human serum.
    Human/Mouse Slit2 Antibody MAB5444

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FAQs for Human/Mouse Slit2 Antibody

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  • Q: Does Human/Mouse Slit2 Antibody (clone # 710305) recognize the whole protein?

    A: The literature states that Slit2 undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. The immunogen sequence used for the antibody is confined to the N-term fragment sequence so we would expect this antibody to bind only the N-term fragment after cleavage. 

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