Slug Antibody (OTI1A6)

Novus Biologicals | Catalog # NBP2-03886

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6
View all Slug Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Slug Antibody (OTI1A6) (NBP2-03886) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-Slug Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Slug Antibody (OTI1A6)

Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]

Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]

Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.
Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]

Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]

Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Human Kidney tissue using anti-SLUG mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] Staining of paraffin-embedded Human lymphoma tissue using anti-SLUG mouse monoclonal antibody.

Applications for Slug Antibody (OTI1A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000
Application Notes
For IHC-P, we recommend using HIER with Tris-EDTA, pH8.0.

Reviewed Applications

Read 1 review rated 5 using NBP2-03886 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Slug

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq].

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Entrez Gene IDs

6591 (Human)

Gene Symbol

SNAI2

Additional Slug Products

Product Documents for Slug Antibody (OTI1A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Slug Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Slug Antibody (OTI1A6)

Customer Reviews for Slug Antibody (OTI1A6) (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse glioma
    Species: Mouse
    Verified Customer | Posted 06/06/2016
    Slug IHC on mouse glioma
    Slug Antibody (OTI1A6) NBP2-03886

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