SMAC/Diablo Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56165

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMAC/Diablo Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human SMAC was used as immunogen.

Reactivity Notes

Cross reacts with Gerbil

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAC/Diablo Antibody - BSA Free

Western Blot: SMAC/Diablo Antibody [NB100-56165]

Western Blot: SMAC/Diablo Antibody [NB100-56165]

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of paraffin-embedded human lung carcinoma using this antibody at dilution of 1:25. Image from verified customer review.
Western Blot: SMAC/Diablo Antibody [NB100-56165]

Western Blot: SMAC/Diablo Antibody [NB100-56165]

Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of MCF7 (A) and K562 (B) cell lysate using this antibody at 1:500.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-56165]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using this antibody at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.
Simple Western: SMAC/Diablo Antibody [NB100-56165]

Simple Western: SMAC/Diablo Antibody [NB100-56165]

Simple Western: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Shows a specific band for Smac in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for SMAC/Diablo Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Simple Western

1:400

Western Blot

1:1000-1:2000
Application Notes
Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:400, apparent MW was 28 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 5 using NB100-56165 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006; Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (approx. 27 kDa), isoform 2 (approx. 22 kDa) and isoform 3 (approx. 21 kDa).

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Entrez Gene IDs

66593 (Mouse); 288753 (Rat)

Gene Symbol

DIABLO

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMAC/Diablo Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for SMAC/Diablo Antibody - BSA Free

Customer Reviews for SMAC/Diablo Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
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1 Stars
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Customer Images

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  • Name: Bryan Tinsley
    Application: Immunohistochemistry-Paraffin
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/29/2014
    IHC analysis of paraffin-embedded human lung carcinoma using Smac antibody at dilution of 1:25.
    SMAC/Diablo Antibody - BSA Free NB100-56165
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/29/2014
    Western blot analysis of extracts from 293 and HeLa cell lines using Smac antibody at dilution of 1:1000
    SMAC/Diablo Antibody - BSA Free NB100-56165

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail