Smad4 Antibody (RM277)

Novus Biologicals | Catalog # NBP2-61587

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM277 expressed in HEK293
View all Smad4 Primary Antibodies »

Product Specifications

Immunogen

A peptide corresponding to the C-terminus of Human Smad4

Reactivity Notes

This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad4 Antibody (RM277)

Western Blot: Smad4 Antibody (RM277) [NBP2-61587]

Western Blot: Smad4 Antibody (RM277) [NBP2-61587]

Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.
Smad4 Antibody (RM277)

Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] -

Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using NBP2-61587 at a 1:5000 dilution.
Smad4 Antibody (RM277)

Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] -

Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61587.

Applications for Smad4 Antibody (RM277)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000-1:2000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Gene Symbol

SMAD4

Additional Smad4 Products

Product Documents for Smad4 Antibody (RM277)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Smad4 Antibody (RM277)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Smad4 Antibody (RM277)

There are currently no reviews for this product. Be the first to review Smad4 Antibody (RM277) and earn rewards!

Have you used Smad4 Antibody (RM277)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies