Smad5 Antibody

Novus Biologicals | Catalog # NBP2-20413

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Smad5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD5. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad5 Antibody

Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: SMAD5 Antibody [NBP2-20413] - SMAD5 antibody detects SMAD5 protein by Western blot analysis. A. 30 ug Raw264. 7 whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract10 % SDS-PAGESMAD5 antibody dilution: 1:1000.
Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: SMAD5 Antibody [NBP2-20413] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: Smad5 Antibody [NBP2-20413]

Western Blot: SMAD5 Antibody [NBP2-20413] - SMAD5 antibody detects SMAD5 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGESMAD5 antibody dilution: 1:1000.
Smad5 Antibody

Western Blot: Smad5 Antibody [NBP2-20413] -

Western Blot: Smad5 Antibody [NBP2-20413] - Smad5 antibody detects smad5 protein by western blot analysis. Various zebrafish tissue extracts (30 ug) were separated by 10 % SDS-PAGE, and the membrane was blotted with Smad5 antibody (NBP2-20413) at a dilution of 1:1000.
Smad5 Antibody

Immunohistochemistry: Smad5 Antibody [NBP2-20413] -

Immunohistochemistry: Smad5 Antibody [NBP2-20413] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Smad5 antibody (NBP2-20413) at 1:300 dilution.

Applications for Smad5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Smad5

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1.

Long Name

Mothers Against DPP Homolog 5

Alternate Names

Dwfc, JV5-1, MADH5

Gene Symbol

SMAD5

UniProt

Q99717

Additional Smad5 Products

Product Documents for Smad5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Smad5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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