SMYD3 Antibody (2P8Z2)

Novus Biologicals | Catalog # NBP3-16102

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2P8Z2 expressed in HEK293
View all SMYD3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SMYD3 (Q9H7B4). SISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMYD3 Antibody (2P8Z2)

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Immunofluorescence analysis of NIH-3T3 cells using SMYD3 Rabbit mAb (NBP3-16102) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
SMYD3 Antibody (2P8Z2)

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] -

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Confocal imaging of NIH/3T3 cells using SMYD3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for SMYD3 Antibody (2P8Z2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMYD3

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Long Name

Histone-lysine N-methyltransferase SMYD3

Alternate Names

ZMYND1, ZNFN3A1

Gene Symbol

SMYD3

Additional SMYD3 Products

Product Documents for SMYD3 Antibody (2P8Z2)

Certificate of Analysis

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Product Specific Notices for SMYD3 Antibody (2P8Z2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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