SOD1/Cu-Zn SOD Antibody

Novus Biologicals | Catalog # NBP2-20534

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Alligator, Avian

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SOD1/Cu-Zn SOD Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD1/Cu-Zn SOD. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody dilution: 1:500.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Rat brain, 15% SDS PAGE gel, diluted at 1:1000.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain. Green: SOD1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: beta Tubulin 3/ TUJ1 antibody diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534]

ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - ELISA detection of SOD1 using SOD1 Antibody for detection at a concentration of 1.5 ug/mL.
SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (NBP2-20534) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SOD1/Cu-Zn SOD Antibody

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

UniProt

P00441

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SOD1/Cu-Zn SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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