Somatostatin Antibody (HL1101) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13719
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1101 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Somatostatin. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Somatostatin Antibody (HL1101) - Azide and BSA Free
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719]
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:250. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat pancreas.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog tissue.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody (HL1101) [NBP3-13719] -
Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at cytoplasmic vesicles and nucleus by immunofluorescent analysis.Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:500.
Red: NF-H, a axon marker, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody (HL1101) [NBP3-13719] -
Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at nucleus and vesicle by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunohistochemistry: Somatostatin Antibody (HL1101) [NBP3-13719] -
Immunohistochemistry: Somatostatin Antibody (HL1101) [NBP3-13719] - Somatostatin antibody [HL1101] detects Somatostatin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Frozen-sectioned mouse brain.
Green: Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:100.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse pancreas.
Green: Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:250.
Red: Vimentin antibody [GT812] diluted at 1:1000.
Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hypothalamus.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Somatostatin Antibody (HL1101) [NBP3-13719] -
Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Somatostatin Antibody (HL1101) [NBP3-13719] -
Somatostatin antibody [HL1101] detects Somatostatin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.
Somatostatin stained by Somatostatin antibody [HL1101] (NBP3-13719) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for Somatostatin Antibody (HL1101) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Somatostatin
Alternate Names
SMST, SOM, SRIF, SST
Gene Symbol
SST
Additional Somatostatin Products
Product Documents for Somatostatin Antibody (HL1101) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Somatostatin Antibody (HL1101) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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