SOX4 Antibody (CL5665) - BSA Free

Novus Biologicals | Catalog # NBP2-61421

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5665

Format

BSA Free
View all SOX4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q06945, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX4 Antibody (CL5665) - BSA Free

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421]

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421]

Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human liver shows absence of nuclear positivity in hepatocytes as expected (negative control).
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong positivity in cells in the rostral migratory stream, as well as in a subset of neurons in the olfactory bulb.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong immunoreactivity in cells in the rostral migratory stream.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse hippocampus shows strong immunoreactivity in a subset of neurons in the granule cell layer of the dentate gyrus.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421]

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.

Applications for SOX4 Antibody (CL5665) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

Additional SOX4 Products

Product Documents for SOX4 Antibody (CL5665) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX4 Antibody (CL5665) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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