SOX4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89506

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOX4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody [NBP1-89506]

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody [NBP1-89506]

Immunocytochemistry/Immunofluorescence: SOX4 Antibody [NBP1-89506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human uterine cervix shows moderate nuclear positivity in a subset of epidermal cells.
SOX4 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody - BSA Free [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody - BSA Free [NBP1-89506]

Staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.
SOX4 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody - BSA Free [NBP1-89506]

Immunohistochemistry-Paraffin: SOX4 Antibody - BSA Free [NBP1-89506]

Staining of human pancreas shows no positivity in glandular cells as expected.
SOX4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SOX4 Antibody - BSA Free [NBP1-89506]

Chromatin Immunoprecipitation-exo-Seq: SOX4 Antibody - BSA Free [NBP1-89506]

ChIP-Exo-Seq composite graph for Anti-SOX4 (NBP1-89506) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SOX4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

Additional SOX4 Products

Product Documents for SOX4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOX4 Antibody - BSA Free

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Protocols

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FAQs

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