Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SOX9/2398
View all SOX9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary) (Uniprot: P48436)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08399)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SOX9 Antibody (SOX9/2398)

Western Blot: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Western Blot: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Western Blot: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Western blot analysis of HepG2 cell lysate using SOX9 antibody (SOX9/2398).
Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human pancreas stained with SOX9 Antibody (SOX9/2398). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human Pancreas stained with SOX9 Mouse Monoclonal Antibody (SOX9/2398).
Protein Array: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Protein Array: SOX9 Antibody (SOX9/2398) [NBP3-07659]

Protein Array: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX9 Mouse Monoclonal Antibody (SOX9/2398).

Applications for SOX9 Antibody (SOX9/2398)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for SOX9 Antibody (SOX9/2398)

Certificate of Analysis

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Product Specific Notices for SOX9 Antibody (SOX9/2398)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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