SP100 Antibody (1G6) - Azide and BSA Free

Novus Biologicals | Catalog # H00006672-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G6

Format

Azide and BSA Free
View all SP100 Primary Antibodies »

Product Specifications

Immunogen

SP100 (NP_003104, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRN

Specificity

SP100 - SP100 nuclear antigen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SP100 Antibody (1G6) - Azide and BSA Free

Western Blot: SP100 Antibody (1G6) [H00006672-M02]

Western Blot: SP100 Antibody (1G6) [H00006672-M02]

Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.
Western Blot: SP100 Antibody (1G6) [H00006672-M02]

Western Blot: SP100 Antibody (1G6) [H00006672-M02]

Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 over-expressed 293 cell line, cotransfected with SP100 Validated Chimera RNAi ( Cat # H00006672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP100 monoclonal antibody (M02), clone 1G6 (Cat # H00006672-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: SP100 Antibody (1G6) [H00006672-M02] - Detection limit for recombinant GST tagged SP100 is 0.03 ng/ml as a capture antibody.

Applications for SP100 Antibody (1G6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP100

SP100 may play a role in the control of gene expression

Alternate Names

DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa

Entrez Gene IDs

6672 (Human)

Gene Symbol

SP100

OMIM

604585 (Human)

UniProt

NP_003104 (Human)

Additional SP100 Products

Product Documents for SP100 Antibody (1G6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SP100 Antibody (1G6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SP100 Antibody (1G6) - Azide and BSA Free

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