SPAM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81637

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPAM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAM1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637] - Analysis in human testis and liver tissues. Corresponding SPAM1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP1-81637] - Staining of human kidney shows no positivity in cells in tubules as expected.

Applications for SPAM1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Long Name

Sperm Adhesion Molecule 1

Alternate Names

HYA1, HYAL3, HYAL5, PH20, SPAG15

Entrez Gene IDs

6677 (Human)

Gene Symbol

SPAM1

UniProt

P38567 (Human)

Additional SPAM1 Products

Product Documents for SPAM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPAM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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