SPDEF Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-04686

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPDEF Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1). MGSASPGLSSVSPSHLLLPPDTVSRTGLEKAAAGAVGLERRDWSPSPPATPEQGLSAFYLSYFDMLYPEDSSWAAKAPGASSREEPPEEPEQCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMVVGEVLKDIETACKLLNITADPMDWSPSNVQKWLLWTEHQYRLPPMGKAFQELAGKELCAMSEEQFRQRSPLGGDVLHAHLDIWKSAAWMKERTSPGAIHYCASTSEESWTDSEVDSSCSGQPIHLWQFLKELLLKPHSYGRFIRWLNKEKGIFKIEDSAQVARLWGIRKNRPAMNYDKLSRSIRQYYKKGIIRKPDISQRLVYQFVHPI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPDEF Antibody - BSA Free

SPDEF Antibody - BSA Free

Western Blot: SPDEF Antibody - BSA Free [SPDEF] -

Western Blot: SPDEF Antibody - BSA Free [SPDEF] - Western blot analysis of lysates from rat ovary, using SPDEF Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
SPDEF Antibody - BSA Free

Immunohistochemistry: SPDEF Antibody - BSA Free [SPDEF] -

Immunohistochemistry: SPDEF Antibody - BSA Free [SPDEF] - Immunohistochemistry analysis of paraffin-embedded human placenta using SPDEF Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
SPDEF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SPDEF Antibody - BSA Free [SPDEF] -

Immunocytochemistry/ Immunofluorescence: SPDEF Antibody - BSA Free [SPDEF] - Immunofluorescence analysis of MCF7 cells using SPDEF Rabbit pAb.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Applications for SPDEF Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPDEF

SPDEF - SAM pointed domain containing ets transcription factor

Alternate Names

bA375E1.3, PDEFRP11-375E1__A.3, Prostate epithelium-specific Ets transcription factor, Prostate-derived Ets factor, Prostate-specific Ets, PSE, SAM pointed domain containing ets transcription factor, SAM pointed domain-containing Ets transcription factor

Gene Symbol

SPDEF

Additional SPDEF Products

Product Documents for SPDEF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPDEF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPDEF Antibody - BSA Free

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