Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30856

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sphingosine Kinase 1/SPHK1 Primary Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with a synthetic peptide derived from the C-terminal of human Sphingosine Kinase 1/SPHK1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Sphingosine Kinase 1/SPHK1 Antibody

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-30856] -

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-30856] - Western blot analysis using Sphingosine Kinase 1/SPHK1 antibody at a dilution of 1:400 on MDBK cell lysate using Novus Biologicals' mouse anti-rabbit HRP secondary antibody at 1:75,000.

Applications for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

: 1:400
Application Notes
.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail