Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Novus Biologicals | Catalog # NBP3-23393

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Sphingosine Kinase 2/SPHK2 Primary Antibodies »

Product Specifications

Immunogen

Phospho-Sphingosine Kinase 2/SPHK2 (Thr-578) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-578 in human Sphingosine Kinase 2/SPHK2. This peptide sequence is highly conserved in rat and mouse Sphingosine Kinase 2/SPHK2 proteins, and is not well conserved in SK1. [UniProt# Q9NRA0]

Modification

p Thr578

Specificity

The antibody detects 70 kDa proteins corresponding to the molecular mass of Sphingosine Kinase 2/SPHK2 on SDS-PAGE immunoblots of human recombinant Sphingosine Kinase 2/SPHK2 and endogenous Sphingosine Kinase 2/SPHK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Western Blot: Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody [NBP3-23393] -

Western Blot: Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody [NBP3-23393] - Western blot of human recombinant Sphingosine Kinase 2/SPHK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-Sphingosine Kinase 2/SPHK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-Sphingosine Kinase 2/SPHK2 (Thr-578) (lanes 3, 4, 7, & 8).

Applications for Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Sphingosine Kinase 2/SPHK2

SPHK2 catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine

Alternate Names

SK2, SPK2

Gene Symbol

SPHK2

Additional Sphingosine Kinase 2/SPHK2 Products

Product Documents for Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

Certificate of Analysis

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Product Specific Notices for Sphingosine Kinase 2/SPHK2 [p Thr578] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail