SPRR3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13374

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPRR3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPRR3 Antibody - BSA Free

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374] - Staining of human uterine cervix shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374] - Staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374] - Staining of human tonsil shows moderate to strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374]

Immunohistochemistry-Paraffin: SPRR3 Antibody [NBP2-13374] - Staining of human liver shows no positivity in hepatocytes as expected.
SPRR3 Antibody - BSA Free Immunohistochemistry: SPRR3 Antibody - BSA Free [NBP2-13374]

Immunohistochemistry: SPRR3 Antibody - BSA Free [NBP2-13374]

Analysis in human esophagus and liver tissues using NBP2-13374 antibody. Corresponding SPRR3 RNA-seq data are presented for the same tissues.

Applications for SPRR3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP2-13374 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRR3

SPRR3, also known as Small proline-rich protein 3, consists of a 169 amino acid isoform that is 18 kDa, and is involved in the surrounding and protection of keratinocytes. Disease research on the protein is currently being conducted with ichthyosis vulgaris, psoriasis, eosinophilic esophagitis, verrucous carcinoma, periodontitis, esophageal cancer, eczema, tonsillitis, and skin disease. SPRR3 is linked to several biological processes, including epidermis development, wound healing, and keratinocyte differentiation, in which it interacts with other proteins, such as IVL, EVPL, NFKBIA, and LOR.

Alternate Names

22 kDa pancornulin, Cornifin beta, Esophagin, small proline-rich protein 3, SPRC

Gene Symbol

SPRR3

Additional SPRR3 Products

Product Documents for SPRR3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SPRR3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPRR3 Antibody - BSA Free

Customer Reviews for SPRR3 Antibody - BSA Free (1)

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  • SPRR3 Antibody
    Name: Felix Oppel
    Application: Indirect Immunofluorescence
    Sample Tested: Cell culture
    Species: Human
    Verified Customer | Posted 05/03/2022
    SPRR3 antibody in indirect immunofluorescence staining of human squamous cell carcinoma cells (green). Nuclei stained with DAPI (blue).
    SPRR3 Antibody - BSA Free NBP2-13374

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Protocols

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