Src [p Tyr419] Antibody (S01-6H3)

Name: Src [p Tyr419] Antibody (S01-6H3)
Brand: Novus Biologicals
SKU: NBP3-19758
Price: 369 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-19758

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-6H3 expressed in HEK293

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Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Tyr419 of human Src (Uniprot # P12931)

Modification

p Tyr419

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Src [p Tyr419] Antibody (S01-6H3) (NBP3-19758) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Src [p Tyr419] Antibody (S01-6H3)

Western Blot-NBP3-19758-Src [p Tyr419] Antibody (S01-6H3)-

Western Blot-NBP3-19758-Src [p Tyr419] Antibody (S01-6H3)-Detection of Phospho-Src (Tyr419) in K562,Hela cell lysates using Phospho-Src (Tyr419) Rabbit mAb(1:1000 diluted).Predicted band size:60kDa.Observed band size:60kDa.

Applications for Src [p Tyr419] Antibody (S01-6H3)

Application

Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

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Product Specific Notices for Src [p Tyr419] Antibody (S01-6H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.