![Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]](https://resources.rndsystems.com/images/products/SRD5A1-Antibody-Immunohistochemistry-Paraffin-NB100-1491-img0002.jpg "Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Guinea Pig
Cited:
Mouse, Guinea Pig
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence ATATGVAEERLLC corresponding to N-Terminus according to NP_001038.1.
Reactivity Notes
Mouse reactivity reported in (PMID: 25797030). Guinea Pig reactivity reported in (PMID: 23585339).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SRD5A1 Antibody
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.![Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]](https://resources.rndsystems.com/images/products/SRD5A1-Antibody-Immunohistochemistry-Paraffin-NB100-1491-img0001.jpg "Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]")
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for SRD5A1 Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
4-6 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
1:100 - 1:2000
Application Notes
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Paraffin embedded Human Liver.
WB usage is reported in scientific literature (PMID: 21747118).
WB usage is reported in scientific literature (PMID: 21747118).
Reviewed Applications
Read 1 review rated 4 using NB100-1491 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SRD5A1
Long Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
Alternate Names
EC 1.3.1.22, S5AR 1, SR type 1
Entrez Gene IDs
6715 (Human)
Gene Symbol
SRD5A1
UniProt
Additional SRD5A1 Products
Product Documents for SRD5A1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SRD5A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SRD5A1 Antibody
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Application: Immunohistochemistry-ParaffinSample Tested: Adrenal gland tissueSpecies: HumanVerified Customer | Posted 10/09/2017Very high immunoexpression in adrenal medulla. High immunoexpression in adrenal cortex.primary Ab diluted 1/50
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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