SSBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-95237

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SSBP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1). MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSBP1 Antibody - BSA Free

Western Blot: SSBP1 AntibodyBSA Free [NBP2-95237]

Western Blot: SSBP1 AntibodyBSA Free [NBP2-95237]

Western Blot: SSBP1 Antibody [NBP2-95237] - Analysis of extracts of Mouse liver, using SSBP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. xposure time: 90s.
Western Blot: SSBP1 AntibodyBSA Free [NBP2-95237]

Western Blot: SSBP1 AntibodyBSA Free [NBP2-95237]

Western Blot: SSBP1 Antibody [NBP2-95237] - Analysis of extracts of various cell lines, using SSBP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: SSBP1 Antibody - BSA Free [NBP2-95237]

Immunoprecipitation: SSBP1 Antibody - BSA Free [NBP2-95237]

Immunoprecipitation: SSBP1 Antibody [NBP2-95237] - Analysis of 200ug extracts of MCF7 cells using 1ug SSBP1 antibody. Western blot was performed from the immunoprecipitate using SSBP1 antibody at a dilition of 1:1000.
SSBP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] -

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] - Confocal immunofluorescence analysis of U2OS cells using SSBP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
SSBP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] -

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] - Immunofluorescence analysis of U2OS cells using SSBP1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SSBP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSBP1

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Mitochondrial single-stranded DNA-binding protein (mtSSB or SSBP1) is necessary for mtDNA replication. SSBP1 is a homotetramer that binds preferentially and cooperatively to single-stranded DNA. It is localized to the mitochondria in normal and neoplastic human tissue of different origin, function, and differentiation, and is likely involved in mitochondrial DNA replication.

Long Name

Single-stranded DNA Binding Protein Motochondrial

Alternate Names

mitochondrial, MtSSB, single-stranded DNA binding protein 1

Gene Symbol

SSBP1

Additional SSBP1 Products

Product Documents for SSBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SSBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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