SSEA-1 Antibody (FUT4/815) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48370

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # FUT4/815

Format

IHC-Prediluted
View all SSEA-1 Primary Antibodies »

Product Specifications

Immunogen

Purified human SSEA-1 (Uniprot: P22083)

Localization

Cell surface and granular paranuclear

Marker

Reed-Sternberg Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for SSEA-1 Antibody (FUT4/815) - IHC-Prediluted

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/815) - IHC-Prediluted [NBP2-48370]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/815) - IHC-Prediluted [NBP2-48370]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/815) - IHC-Prediluted [NBP2-48370] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Mouse Monoclonal Antibody (FUT4/815).

Applications for SSEA-1 Antibody (FUT4/815) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 11q21 Molecular weight of antigen: ~220kDa (Glycoprotein) Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (FUT4/815) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for SSEA-1 Antibody (FUT4/815) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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