SSX3 Antibody (4A11) - Azide and BSA Free

Novus Biologicals | Catalog # H00010214-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A11

Format

Azide and BSA Free
View all SSX3 Primary Antibodies »

Product Specifications

Immunogen

SSX3 (NP_066294, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNGDDTFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKVSEKIVYVYMKRKYEAMTKLGFKAILPSFMRNKRVTDFQGNDFDNDPNRGNQVQRPQMT

Specificity

Reacts with synovial sarcoma, X breakpoint 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SSX3 Antibody (4A11) - Azide and BSA Free

Western Blot: SSX3 Antibody (4A11) [H00010214-M03]

Western Blot: SSX3 Antibody (4A11) [H00010214-M03]

Western Blot: SSX3 Antibody (4A11) [H00010214-M03] - Analysis of SSX3 expression in transfected 293T cell line by SSX3 monoclonal antibody (M03), clone 4A11. Lane 1: SSX3 transfected lysatE (21.697 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: SSX3 Antibody (4A11) [H00010214-M03] - Detection limit for recombinant GST tagged SSX3 is 0.3 ng/ml as a capture antibody.

Applications for SSX3 Antibody (4A11) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SSX3

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This gene appears not to be involved in this type of chromosome translocation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

CT5.3Cancer/testis antigen 5.3, MGC119054, MGC14495, protein SSX3, synovial sarcoma, X breakpoint 3

Entrez Gene IDs

10214 (Human)

Gene Symbol

SSX3

UniProt

NP_066294 (Human)

Additional SSX3 Products

Product Documents for SSX3 Antibody (4A11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SSX3 Antibody (4A11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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