StAR Antibody (5F9) - Azide and BSA Free

Novus Biologicals | Catalog # H00006770-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5F9

Format

Azide and BSA Free
View all StAR Primary Antibodies »

Product Specifications

Immunogen

STAR (AAH10550, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVG

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22708737).

Localization

Mitochondrial

Specificity

STAR - steroidogenic acute regulator

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for StAR Antibody (5F9) - Azide and BSA Free

Western Blot: StAR Antibody (5F9) [H00006770-M01]

Western Blot: StAR Antibody (5F9) [H00006770-M01]

Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: StAR Antibody (5F9) [H00006770-M01]

Western Blot: StAR Antibody (5F9) [H00006770-M01]

Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: StAR Antibody (5F9) [H00006770-M01] - Detection limit for recombinant GST tagged STAR is approximately 0.03ng/ml as a capture antibody.

Applications for StAR Antibody (5F9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: StAR

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Alternate Names

cholesterol trafficker, mitochondrial steroid acute regulatory protein, StAR, StARD1, STARD1StAR-related lipid transfer (START) domain containing 1, START domain containing 1, START domain-containing protein 1, steroid acute regulatory protein, steroidogenic acute regulator, steroidogenic acute regulatory protein, steroidogenic acute regulatory protein, mitochondrial

Entrez Gene IDs

6770 (Human)

Gene Symbol

STAR

OMIM

201710 (Human)

UniProt

AAH10550 (Human)

Additional StAR Products

Product Documents for StAR Antibody (5F9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for StAR Antibody (5F9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for StAR Antibody (5F9) - Azide and BSA Free

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