Strep II Tag Antibody (517) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43735

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 517

Format

Azide and BSA Free
View all Strep II Tag Primary Antibodies »

Product Specifications

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Strep II Tag Antibody (517) - Azide and BSA Free

Western Blot: Strep II Tag Antibody (517) [NBP2-43735]

Western Blot: Strep II Tag Antibody (517) [NBP2-43735]

Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.
Western Blot: Strep II Tag Antibody (517) [NBP2-43735]

Western Blot: Strep II Tag Antibody (517) [NBP2-43735]

Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0004.jpg
Strep II Tag Antibody (517) - Azide and BSA Free

Western Blot: Strep II Tag Antibody (517) [NBP2-43735] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Strep II Tag Antibody (517) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (517) [NBP2-43735] -

Strep epitope Tag antibody [GT517] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

Applications for Strep II Tag Antibody (517) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Reviewed Applications

Read 2 reviews rated 5 using NBP2-43735 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Documents for Strep II Tag Antibody (517) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Strep II Tag Antibody (517) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Strep II Tag Antibody (517) - Azide and BSA Free

Customer Reviews for Strep II Tag Antibody (517) - Azide and BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 2 of 2 reviews Showing All
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  • Strep II Tag Antibody (517)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA MB 231 cells and Human breast cell lysate (MDA-MB-231)
    Species: Human
    Verified Customer | Posted 01/15/2018
    Strep II Tag Antibody (517) - Azide and BSA Free NBP2-43735
  • Strep II Tag Antibody (517)
    Name: Michal Dibus
    Application: Immunoprecipitation
    Sample Tested: Immunoprecipitation from cell lysate, Western blot and MDA-231 cell lysate
    Species: Human
    Verified Customer | Posted 05/26/2017
    Immunoprecipitation of StrepII-tagged protein: MDA-MB-231 cells were transfected with StrepII-tagged protein or empty vector for control. For immunoprecipitation, 1ug of StrepII (517) antibody was crosslinked to Protein G sepharose with DMP (dimethyl pimelimidate) and incubated with cleared lysate in 4°C for 3h. For control, empty Protein G sepharose was used. The samples were resolved using SDS-PAGE and immunoblot with StrepII (517) antibody.
    Strep II Tag Antibody (517) - Azide and BSA Free NBP2-43735

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