Strep II Tag Antibody (661) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43719

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 661

Format

Azide and BSA Free
View all Strep II Tag Primary Antibodies »

Product Specifications

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Strep II Tag Antibody (661) - Azide and BSA Free

Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719]

Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719]

Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Strep II Tag Antibody (661) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719] -

Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.
Strep II Tag Antibody (661) - Azide and BSA Free

Western Blot: Strep II Tag Antibody (661) [NBP2-43719] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (NBP2-43719) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for Strep II Tag Antibody (661) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Documents for Strep II Tag Antibody (661) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Strep II Tag Antibody (661) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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