Strep II Tag Antibody (HL1252) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25699

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1252 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to Strep II Tag.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Strep II Tag Antibody (HL1252) - Azide and BSA Free

Strep II Tag Antibody (HL1252) - Azide and BSA Free

Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] -

Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 ug of Strep epitope Tag antibody [HL1252] (NBP3-25699). Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (NBP3-25699). EasyBlot HRP-conjugated anti rabbit IgG antibody.
Strep II Tag Antibody (HL1252) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] -

Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (NBP3-25699) diluted at 1:500. Blue: Fluoroshield with DAPI.
Strep II Tag Antibody (HL1252) - Azide and BSA Free

Western Blot: Strep II Tag Antibody (HL1252) [NBP3-25699] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep II Tag antibody [HL1252] (NBP3-25699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Strep II Tag Antibody (HL1252) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Strep II Tag Antibody (HL1252) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Strep II Tag Antibody (HL1252) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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