SUMO Activating Enzyme E1 (SAE1) Antibody

Novus Biologicals | Catalog # NB100-1312

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SUMO Activating Enzyme E1 (SAE1/UBA2) Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence MKGNGIVECLGPK corresponding to C-Terminus according to NP_005491.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NB100-1312]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NB100-1312]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NB100-1312] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for SUMO Activating Enzyme E1 (SAE1) Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in Jurkat and 293 lysates (predicted MW of 40 kDa band according to NP_005491).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

SAE1, or SUMO-1 (small ubiquitin-like modifier, ~12kDa), is a ubiquitin-like (Ubl) protein that has been shown to bind to several protiens including Ran GTPase-activating protein 1 (RanGAP1), IkappaBalpha, and PML. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes.

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Entrez Gene IDs

10055 (Human)

Gene Symbol

SAE1

UniProt

NP_005491. (Human)

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SUMO Activating Enzyme E1 (SAE1) Antibody

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