SUMO Activating Enzyme E1 (SAE1) Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-44220

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SUMO Activating Enzyme E1 (SAE1/UBA2) Primary Antibodies »

Product Specifications

Immunogen

This purified SUMO Activating Enzyme E1 (SAE1) Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 (SAE1) (SAE1) protein. (Uniprot: Q9UBE0)

Reactivity Notes

A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This purified antibody is directed against human SUMO Activating Enzyme E1 (SAE1) protein. The product was purified from monospecific antiserum by affinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody - BSA Free

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220] - Left lane (-): 20 ug human HeLa whole cell protein. Right lane (+): 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with SUMO Activating Enzyme E1 (SAE1) Antibody (2 ug/mL) at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.

Applications for SUMO Activating Enzyme E1 (SAE1) Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Western Blot

2ug/ml
Application Notes
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Gene Symbol

SAE1

UniProt

Q9UBE0 (Human)

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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