Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SUMO2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SUMO2 Antibody - Azide and BSA Free

Western Blot: SUMO2 Antibody [NBP2-98860]

Western Blot: SUMO2 Antibody [NBP2-98860]

Western Blot: SUMO2 Antibody [NBP2-98860] - Lane A: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: SUMO2 Antibody [NBP2-98860]

Immunoprecipitation: SUMO2 Antibody [NBP2-98860]

Immunoprecipitation: SUMO2 Antibody [NBP2-98860] - Lane A:0.5 mg SW480 Whole Cell Lysate2 uL anti-SUMO2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUMO2 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa
SUMO2 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SUMO2 Antibody [NBP2-98860] -

Immunocytochemistry/Immunofluorescence: SUMO2 Antibody [NBP2-98860] - Immunofluorescence staining of SUMO2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98860 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus..

Applications for SUMO2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUMO2

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4; Subcellular location: Nucleus

Long Name

Small Ubiquitin-like Modifier 2

Alternate Names

Sentrin-2, SMT3B, SMT3H2

Gene Symbol

SUMO2

Additional SUMO2 Products

Product Documents for SUMO2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SUMO2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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