Suppressor of Fused Antibody (HL3375)

Novus Biologicals | Catalog # NBP3-48810

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3375
View all Suppressor of Fused Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Suppressor of Fused.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Suppressor of Fused Antibody (HL3375)

Suppressor of Fused Antibody (HL3375)

Western Blot: Suppressor of Fused Antibody (HL3375) [NBP3-48810] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (NBP3-48810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Suppressor of Fused Antibody (HL3375)

Western Blot: Suppressor of Fused Antibody (HL3375) [NBP3-48810] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (NBP3-48810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Suppressor of Fused Antibody (HL3375)

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody (HL3375) [NBP3-48810] -

Suppressor of Fused antibody [HL3375] detects Suppressor of Fused protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Suppressor of Fused stained by Suppressor of Fused antibody [HL3375] (NBP3-48810) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for Suppressor of Fused Antibody (HL3375)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Suppressor of Fused

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Alternate Names

SUFUHSUFUXLPRO1280, suppressor of fused homolog, suppressor of fused homolog (Drosophila)

Gene Symbol

SUFU

Additional Suppressor of Fused Products

Product Documents for Suppressor of Fused Antibody (HL3375)

Certificate of Analysis

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Product Specific Notices for Suppressor of Fused Antibody (HL3375)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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