Suppressor of Fused Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87384

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Suppressor of Fused Antibody - BSA Free

Western Blot: Suppressor of Fused Antibody [NBP1-87384]

Western Blot: Suppressor of Fused Antibody [NBP1-87384]

Western Blot: Suppressor of Fused Antibody [NBP1-87384] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP1-87384]

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP1-87384]

Immunocytochemistry/Immunofluorescence: Suppressor of Fused Antibody [NBP1-87384] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Tonsil shows moderate nuclear positivity in germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384]

Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.

Applications for Suppressor of Fused Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Suppressor of Fused

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Alternate Names

SUFUHSUFUXLPRO1280, suppressor of fused homolog, suppressor of fused homolog (Drosophila)

Gene Symbol

SUFU

Additional Suppressor of Fused Products

Product Documents for Suppressor of Fused Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Suppressor of Fused Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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