SUR1 Antibody (S289-16) - BSA Free

Novus Biologicals | Catalog # NBP2-59320

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S289-16

Format

BSA Free
View all SUR1 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1

Localization

Membrane

Specificity

Detects 160kDa. Does not cross-react with SUR2B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SUR1 Antibody (S289-16) - BSA Free

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320]

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320]

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.
Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.
Western Blot: SUR1 Antibody (S289-16) [NBP2-59320]

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320]

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320]

SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg
SUR1 Antibody (S289-16)

Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] -

Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SUR1 Antibody (S289-16) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 4 using NBP2-59320 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SUR1

SUR1 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternative splicing of this gene has been observed; however, the transcript variants have not been fully described.

Long Name

ATP-binding cassette sub-family C member 8

Alternate Names

ABCC8, HRINS, SUR

Gene Symbol

ABCC8

Additional SUR1 Products

Product Documents for SUR1 Antibody (S289-16) - BSA Free

Certificate of Analysis

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Product Specific Notices for SUR1 Antibody (S289-16) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SUR1 Antibody (S289-16) - BSA Free

Customer Reviews for SUR1 Antibody (S289-16) - BSA Free (2)

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  • SUR1 Antibody (S289-16)
    Name: Luiz Barella
    Application: Western Blot
    Sample Tested: Mouse pancreatic islets
    Species: Mouse
    Verified Customer | Posted 02/25/2019
    Western Blot analysis of mouse pancreatic islets using Mouse Anti-SUR1 S289-16. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10 ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16 hours at 4 degrees celsius. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT.
    SUR1 Antibody (S289-16) - BSA Free NBP2-59320
  • SUR1 Antibody (S289-16)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Tissue Sections - Pancreas
    Species: Human
    Verified Customer | Posted 07/07/2017
    Immunohistochemistry-Paraffin: SUR1 Antibody (S289-16) [NBP2-59320]
    SUR1 was detected in immersion fixed paraffin-embedded sections of normal human pancreas using citrate pH6 pretreatment and incubating at 1:50 dilution for 30 minutes, with LabVision Quanto Detection.
    SUR1 Antibody (S289-16) - BSA Free NBP2-59320

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