SV2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82964

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SV2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECGHGRFQW

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SV2A Antibody - BSA Free

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex and small intestine tissues using HPA007863 antibody. Corresponding SV2A RNA-seq dat
Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NBP1-82964]

Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NBP1-82964]

Immunocytochemistry/Immunofluorescence: SV2A Antibody [NBP1-82964] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human small intestine shows no positivity in glandular cells.
Western Blot: SV2A Antibody [NBP1-82964]

Western Blot: SV2A Antibody [NBP1-82964]

Western Blot: SV2A Antibody [NBP1-82964] - Analysis of SV2A in (1) undifferentiated SH-SY5Y, (2) differentiated SH-SY5Y (neurons) and (3) mouse brain using anti-SV2A antibody. 30ug of protein/lane. Image from verified customer review.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964]

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunoprecipitation: SV2A Antibody [NBP1-82964]

Immunoprecipitation: SV2A Antibody [NBP1-82964]

Immunoprecipitation: SV2A Antibody [NBP1-82964] - Staining of neuroblastoma cells SH-SY5Y differentiated into neurons. Lane (1) 10ug of input, 500ug used for IP. 10ul of antibody used for incubation with lysate (overnight incubation at 4C with rotation) prior to addition of beads. Image from verified customer review.

Applications for SV2A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Immunoprecipitation

Reported in scientific literature (PMID: 24284412)/verified customer review.

Western Blot

Reported in scientific literature (PMID: 24284412)/verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-82964 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SV2A

SV2A (Synaptic Vesicle protein 2A) is an integral membrane glycoproteins present in synaptic vesicles. SV2s have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Long Name

Synaptic Vesicle Glycoprotein 2A

Alternate Names

KIAA0736, SV2

Gene Symbol

SV2A

Additional SV2A Products

Product Documents for SV2A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SV2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SV2A Antibody - BSA Free

Customer Reviews for SV2A Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Jennifer Fox
    Application: Western Blot
    Sample Tested: L1: undifferentiated SH-SY5Y, L2: differentiated SH-Sy5Y (neurons), L3: Mouse Brain
    Species: Mouse and Human
    Verified Customer | Posted 01/28/2015
    SV2A Western Blot
    SV2A Antibody - BSA Free NBP1-82964
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Neuroblastoma cells SH-SY5Y differentiated into neurons
    Species: Human
    Verified Customer | Posted 01/28/2015
    SV2A IP
    SV2A Antibody - BSA Free NBP1-82964

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Protocols

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FAQs

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