Synapsin II Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13443
![Western Blot: Synapsin II Antibody [NBP3-13443]](https://resources.rndsystems.com/images/products/Synapsin-II-Antibody-Western-Blot-NBP3-13443-img0002.jpg "Western Blot: Synapsin II Antibody [NBP3-13443]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Synapsin II. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Synapsin II Antibody - BSA Free
Western Blot: Synapsin II Antibody [NBP3-13443]
Western Blot: Synapsin II Antibody [NBP3-13443] - Mouse tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13443) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Synapsin II Antibody [NBP3-13443]](https://resources.rndsystems.com/images/products/Synapsin-II-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13443-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Synapsin II Antibody [NBP3-13443]")
Immunocytochemistry/ Immunofluorescence: Synapsin II Antibody [NBP3-13443]
Immunocytochemistry/Immunofluorescence: Synapsin II Antibody [NBP3-13443] - Synapsin II antibody detects Synapsin II protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synapsin II stained by Synapsin II antibody (NBP3-13443) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.![Western Blot: Synapsin II Antibody [NBP3-13443]](https://resources.rndsystems.com/images/products/Synapsin-II-Antibody-Western-Blot-NBP3-13443-img0001.jpg "Western Blot: Synapsin II Antibody [NBP3-13443]")
Western Blot: Synapsin II Antibody [NBP3-13443]
Western Blot: Synapsin II Antibody [NBP3-13443] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13443) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Synapsin II Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synapsin II
Alternate Names
synapsin IISYNII, synapsin-2, SYNIIa, SYNIIb
Gene Symbol
SYN2
Additional Synapsin II Products
Product Documents for Synapsin II Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Synapsin II Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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