Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rSYP/6856

Format

Azide and BSA Free
View all Synaptophysin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Localization

Cell junction, Cytoplasmic vesicle, Secretory vesicle, Synapse, Synaptic vesicle membrane, Synaptosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free

Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free

Immunohistochemistry-Paraffin: Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free [NBP3-49530] -

Formalin-fixed, paraffin-embedded human brain stained with Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary. Secondary antibody negative control.

Applications for Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin (Syp I), also called the major synaptic vesicle protein p38, is an integral membrane glycoprotein found in the synaptic vesicles of brain, neuron and neuroendocrine cells. Synaptophysin is one of the most abundant membrane proteins, comprising approximately 7% of the total protein in small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. Synaptophysin may also be used as a neuroendocrine tumor marker in both neuroscience and cancer research.

Alternate Names

SYP

Gene Symbol

SYP

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Synaptophysin Antibody (rSYP/6856) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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