SYNE1 Antibody (1D4D9)

Name: SYNE1 Antibody (1D4D9)
Brand: Novus Biologicals
SKU: NBP3-15911
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15911

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1D4D9 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 8200-8400 of human SYNE1 (Q8NF91). FGRVERYHKKLIRLPLPDDEHDLSDRELELEDSAALSDLHWHDRSADSLLSPQPSSNLSLSLAQPLRSERSGRDTPASVDSIPLEWDHDYDLSRDLESAMSRALPSEDEEGQDDKDFYLRGAVGLSGDHSALESQIRQLGKALDDSRFQIQQTENIIRSKTPTGPELDTSYKGYMKLLGECSSSIDSVKRLEHKLKEEEES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SYNE1 Antibody (1D4D9)

Immunocytochemistry/ Immunofluorescence: SYNE1 Antibody (1D4D9) [NBP3-15911]

Immunocytochemistry/Immunofluorescence: SYNE1 Antibody (1D4D9) [NBP3-15911] - Immunofluorescence analysis of NIH-3T3 cells using SYNE1 Rabbit mAb (NBP3-15911) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: SYNE1 Antibody (1D4D9) [NBP3-15911] -

Western Blot: SYNE1 Antibody (1D4D9) [NBP3-15911] - Western blot analysis of various lysates using SYNE1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for SYNE1 Antibody (1D4D9)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SYNE1

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity. There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity.

Alternate Names

ARCA1, C6orf98, CPG2, dJ45H2.2, EC 4.2.1.49, EC 4.3.1.3, EDMD4, Enaptin, KIAA0796chromosome 6 open reading frame 98, KIAA1262FLJ41140, KIAA17568B, Myne-1, MYNE1DKFZp781J13156, Myocyte nuclear envelope protein 1, nesprin 1, nesprin-1, Nuclear envelope spectrin repeat protein 1, SCAR8FLJ30878, spectrin repeat containing, nuclear envelope 1, Synaptic nuclear envelope protein 1, synaptic nuclei expressed gene 1, Syne-1, SYNE-1B

Gene Symbol

SYNE1

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Product Documents for SYNE1 Antibody (1D4D9)

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Certificate of Analysis

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Product Specific Notices for SYNE1 Antibody (1D4D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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