Syntaxin-BP1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20526
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Syntaxin-BP1. The exact sequence is proprietary.
Localization
Cytoplasm; Membrane; Peripheral membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Syntaxin-BP1 Antibody - BSA Free
Western Blot: Syntaxin-BP1 Antibody [NBP2-20526]
Western Blot: STXBP1 Antibody [NBP2-20526] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:3000.Immunocytochemistry/ Immunofluorescence: Syntaxin-BP1 Antibody [NBP2-20526]
Immunocytochemistry/Immunofluorescence: STXBP1 Antibody [NBP2-20526] - Confocal immunofluorescence analysis of methanol-fixed A431, using STXBP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.Western Blot: Syntaxin-BP1 Antibody [NBP2-20526] -
Western Blot: Syntaxin-BP1 Antibody [NBP2-20526] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Syntaxin-BP1 antibody (NBP2-20526) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Syntaxin-BP1 Antibody [NBP2-20526] -
Munc18-1 antibody detects Munc18-1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Munc18-1 protein stained by Munc18-1 antibody (NBP2-20526) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for Syntaxin-BP1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Syntaxin-BP1
Long Name
Syntaxin-binding Protein 1
Alternate Names
EIEE4, p67, rbSec1, STXBP1, SyntaxinBP1, UNC18
Gene Symbol
STXBP1
Additional Syntaxin-BP1 Products
Product Documents for Syntaxin-BP1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Syntaxin-BP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for Syntaxin-BP1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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