Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74422

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H10

Format

Azide and BSA Free
View all Syntrophin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SNTA1 (NP_003089) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free

Western Blot: Syntrophin alpha 1 Antibody (OTI1H10)Azide and BSA Free [NBP2-74422]

Western Blot: Syntrophin alpha 1 Antibody (OTI1H10)Azide and BSA Free [NBP2-74422]

Western Blot: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntrophin alpha 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syn
Immunocytochemistry/ Immunofluorescence: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Immunocytochemistry/ Immunofluorescence: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Immunocytochemistry/Immunofluorescence: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Syntrophin alpha 1.
Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Syntrophin alpha 1 antibody, and then analyzed by flow cytometry.
Western Blot: Syntrophin alpha 1 Antibody (OTI1H10)Azide and BSA Free [NBP2-74422]

Western Blot: Syntrophin alpha 1 Antibody (OTI1H10)Azide and BSA Free [NBP2-74422]

Western Blot: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Syntrophin alpha 1 monoclonal antibody.
Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422]

Flow Cytometry: Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free [NBP2-74422] - Analysis of Hela cells, using anti-Syntrophin alpha 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntrophin alpha 1

Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes.

Alternate Names

59 kDa dystrophin-associated protein A1 acidic component 1, dJ1187J4.5, LQT12, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, SNT1alpha-1-syntrophin, syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component), syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component), syntrophin-1, TACIP1acidic alpha 1 syntrophin

Gene Symbol

SNTA1

Additional Syntrophin alpha 1 Products

Product Documents for Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Syntrophin alpha 1 Antibody (OTI1H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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