T Cell Exhaustion Marker (Flow) Antibody Pack

Novus Biologicals | Catalog # NBP3-12864

Novus Biologicals
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Key Product Details

Species

Human

Applications

CyTOF-ready, Flow Cytometry

Product Summary for T Cell Exhaustion Marker (Flow) Antibody Pack

The Human T Cell Exhaustion Marker Sample Pack (Flow) contains antibodies to detect expression of inhibitory receptors associated with T cell exhaustion including PD-1, CTLA-4, TIGIT, TIM-3, LAG-3 and VISTA. Immune cells expressing these inhibitory receptors are found during chronic viral infection and within the tumor microenvironment of many solid tumors. Also included are three PE-Conjugated secondary antibodies for fluorescent detection by flow cytometry.All antibodies have been validated for use in flow cytometry applications.

This pack contains 1 vial each of:
MAB10863-SP (25 ug):
MAB3253-SP (25 ug)
MAB7898-SP (25 ug)
MAB2365R-SP (25 ug)
MAB71263-SP (25 ug)
MAB23191-SP (25 ug)
F0110 (25ug)
(100 Tests)
F0102B (100 Tests)
F0105B (100 Tests)

Product Specifications

Specificity

Please see each vial's data sheet for specificity information specific to each product.

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Marker

T cell exhaustion

Scientific Data Images for T Cell Exhaustion Marker (Flow) Antibody Pack

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog# FAB2408P). Quadrant markers were set based on control antibody staining (Catalog# MAB0041).
Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog#: FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog# MAB6944or (B) Normal Rabbit IgG Control (Catalog# AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0110) at 10 uL/10^6 cells.
Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) - Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 ng/mL PHA for 2 days or (B) untreated, were stained with Rabbit Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10863) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# F0111) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog# FAB100P). Quadrant markers were set based on control antibody staining (Catalog# MAB1050).
Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) - NS0 mouse myeloma cell line transfected with human CTLA-4 and eGFP was stained with (A) Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) or (B) Normal Rabbit IgG Control Antibody (Catalog# MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog# F0111).
Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) Antibody Pack [NBP3-12864]

Flow Cytometry: T Cell Exhaustion Marker (Flow) - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Kit Contents for T Cell Exhaustion Marker (Flow) Antibody Pack

Formulation, Preparation, and Storage

Formulation

See individual datasheets

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage is content dependent.

Background: T Cell Exhaustion Marker (Flow)

Alternate Names

exhaustion markers, immune cell exhaustion, immune checkpoint markers, inhibitory receptors, T cell exhaustion

Additional T Cell Exhaustion Marker (Flow) Products

Product Documents for T Cell Exhaustion Marker (Flow) Antibody Pack

Certificate of Analysis

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Product Specific Notices for T Cell Exhaustion Marker (Flow) Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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