T7 Epitope Tag Antibody [Biotin]

Novus Biologicals | Catalog # NB600-371B

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all T7 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for T7 Epitope Tag Antibody [Biotin]

Western Blot: T7 Epitope Tag Antibody [Biotin] [NB600-371B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 10 seconds.

Applications for T7 Epitope Tag Antibody [Biotin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: T7 Epitope Tag

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Alternate Names

MASMTGGQQMG epitope tag, MASMTGGQQMG tag, T7 epitope tag

Additional T7 Epitope Tag Products

Product Documents for T7 Epitope Tag Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for T7 Epitope Tag Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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