T7 Epitope Tag Antibody [HRP]

Novus Biologicals | Catalog # NB600-377

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative)

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all T7 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for T7 Epitope Tag Antibody [HRP]

Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for T7 Epitope Tag Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000-1:100000

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.05% Pro-Clean 400

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: T7 Epitope Tag

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Alternate Names

MASMTGGQQMG epitope tag, MASMTGGQQMG tag, T7 epitope tag

Additional T7 Epitope Tag Products

Product Documents for T7 Epitope Tag Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for T7 Epitope Tag Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for T7 Epitope Tag Antibody [HRP]

There are currently no reviews for this product. Be the first to review T7 Epitope Tag Antibody [HRP] and earn rewards!

Have you used T7 Epitope Tag Antibody [HRP]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies